s infantis atcc baa 1675 (ATCC)

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ATCC s infantis atcc baa 1675
S Infantis Atcc Baa 1675, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/s+infantis+atcc+baa+1675/Salmonella+enterica%3B+subsp%2E+enterica%3B+serovar+Infantis/pmc12752171-94-12-14
Average 93 stars, based on 18 article reviews

s infantis atcc baa 1675 - by Bioz Stars, 2026-10

93/100 stars

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Incubation:

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis.
Article Snippet: Later, an aliquot of 10 mL sample was concentrated through 122 a 100 kDa cut-off Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, 123 Billerica, MA, USA). .. An aliquot of 50 L concentrated sample was mixed with 124 100 L overnight culture of S. Infantis ATCC BAA-1675 and 2L CaCl2 and 125 then incubated at room temperature (25C) for 5 minutes before conducting 126 a double-layer plaque assay [16]. .. After incubating at 37C overnight, a single 127 large plaque was picked and resuspended in 1 mL of SM buffer (Thermo 128 Fisher Scientific, Waltham, MA, USA), followed by another round of the AR TIC LE IN PR ES S 7 129 double-layer plaque assay (known as single-plaque purification).

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis
Article Snippet: Later, an aliquot of 10 mL sample was concentrated through a 100 kDa cut-off Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, Billerica, MA, USA). .. An aliquot of 50 μL concentrated sample was mixed with 100 μL overnight culture of S. Infantis ATCC BAA-1675 and 2μL CaCl 2 and then incubated at room temperature (25 °C) for 5 min before conducting a double-layer plaque assay [ ]. .. After incubating at 37 °C overnight, a single large plaque was picked and resuspended in 1 mL of SM buffer (Thermo Fisher Scientific, Waltham, MA, USA), followed by another round of the double-layer plaque assay (also known as single-plaque purification).

Plaque Assay:

Purification:

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis.
Article Snippet: .. 132 After purification, the phage was propagated with the overnight 133 bacterial culture of S. Infantis ATCC BAA-1675 in 40 mL tryptic soy broth 134 (TSB; Difco, Becton Dickinson, Sparks, MD, USA), supplemented with CaCl2 135 at a final concentration of 10 mM, at 37C for 24 hours. ..

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis
Article Snippet: .. After purification, the phage was propagated with the overnight bacterial culture of S. Infantis ATCC BAA-1675 in 40 mL tryptic soy broth (TSB; Difco, Becton Dickinson, Sparks, MD, USA), supplemented with CaCl 2 at a final concentration of 10 mM, at 37 °C for 24 h. Subsequently, the phage lysate was centrifuged at 8,000 × g for 5 min before 0.22-μm filtration to remove bacterial debris for further concentration and antimicrobial activity test(s). .. The phage lysate was further concentrated via a 50 kDa cut-off Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, Billerica, MA, USA) and purified via cesium chloride (CsCl) gradient ultracentrifugation to remove bacterial debris, as previously described [ ], before downstream analysis, such as phage morphology observation, proteomic analysis, and DNA extraction for whole genome sequencing.

Concentration Assay:

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis.
Article Snippet: .. 132 After purification, the phage was propagated with the overnight 133 bacterial culture of S. Infantis ATCC BAA-1675 in 40 mL tryptic soy broth 134 (TSB; Difco, Becton Dickinson, Sparks, MD, USA), supplemented with CaCl2 135 at a final concentration of 10 mM, at 37C for 24 hours. ..

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis
Article Snippet: .. After purification, the phage was propagated with the overnight bacterial culture of S. Infantis ATCC BAA-1675 in 40 mL tryptic soy broth (TSB; Difco, Becton Dickinson, Sparks, MD, USA), supplemented with CaCl 2 at a final concentration of 10 mM, at 37 °C for 24 h. Subsequently, the phage lysate was centrifuged at 8,000 × g for 5 min before 0.22-μm filtration to remove bacterial debris for further concentration and antimicrobial activity test(s). .. The phage lysate was further concentrated via a 50 kDa cut-off Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, Billerica, MA, USA) and purified via cesium chloride (CsCl) gradient ultracentrifugation to remove bacterial debris, as previously described [ ], before downstream analysis, such as phage morphology observation, proteomic analysis, and DNA extraction for whole genome sequencing.

Filtration:

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis
Article Snippet: .. After purification, the phage was propagated with the overnight bacterial culture of S. Infantis ATCC BAA-1675 in 40 mL tryptic soy broth (TSB; Difco, Becton Dickinson, Sparks, MD, USA), supplemented with CaCl 2 at a final concentration of 10 mM, at 37 °C for 24 h. Subsequently, the phage lysate was centrifuged at 8,000 × g for 5 min before 0.22-μm filtration to remove bacterial debris for further concentration and antimicrobial activity test(s). .. The phage lysate was further concentrated via a 50 kDa cut-off Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, Billerica, MA, USA) and purified via cesium chloride (CsCl) gradient ultracentrifugation to remove bacterial debris, as previously described [ ], before downstream analysis, such as phage morphology observation, proteomic analysis, and DNA extraction for whole genome sequencing.

Activity Assay:

Article Title: A Tequintavirus bacteriophage SIA3lw isolated from sewage water with antimicrobial potential against antibiotic-resistant Salmonella Infantis
Article Snippet: .. After purification, the phage was propagated with the overnight bacterial culture of S. Infantis ATCC BAA-1675 in 40 mL tryptic soy broth (TSB; Difco, Becton Dickinson, Sparks, MD, USA), supplemented with CaCl 2 at a final concentration of 10 mM, at 37 °C for 24 h. Subsequently, the phage lysate was centrifuged at 8,000 × g for 5 min before 0.22-μm filtration to remove bacterial debris for further concentration and antimicrobial activity test(s). .. The phage lysate was further concentrated via a 50 kDa cut-off Amicon Ultra-15 Centrifugal Filter Unit (Merck Millipore, Billerica, MA, USA) and purified via cesium chloride (CsCl) gradient ultracentrifugation to remove bacterial debris, as previously described [ ], before downstream analysis, such as phage morphology observation, proteomic analysis, and DNA extraction for whole genome sequencing.

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